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 38 years of experience in the manufacturing of IEF carrier ampholytes, e.g. Sepalytes and GelStick PAG film, and sustained efforts to optimise IEF separation performance have led to the new product we call

ProPhyl Air Gel Matrix

 –NEW–
 A novel gel matrix for native protein electrophoresis
–NEW– 

ProPhyl Air is a new type of gel matrix with unusual physical properties which, in combination with our self-produced carrier ampholyte (Sepalytes), represents an excellent resolution of native protein mixtures in isoelectric focusing. ProPhyl Air improves the solubility of proteins because it carries charged groups. This not only affects entry of proteins into the gel but also prevent precipitation near the isoelectric point, which gives rise to sharper bands in general and also more bands.

Its use could therefore be revolutionary for those working with a wide range of proteins

To convince you of the excellent separation properties of ProPhyl Air, simply send us your native protein samples.
Within 2-4 days we will send you the results at no cost!
(Please contact: m.demharter@protec-biosep.de)

ProPhyl Air stands for : protein loving, open structured

 

Multiple sclerosis (MS)
A chronic autoimmune disorder characterized by inflammatory-demyelinating
events in the central nervous system

 

Parkinson’s disease (PD)
A progressive nervous system disorder that affects movement



(alpha-synuclein in cerebrospinal fluid)

The diagnosis of multiple sclerosis in the cerebrospinal fluid is carried out with the help of ProPhyl Air and the corresponding Sepalyte CSF by isoelectric focusing
– New –
(horizontal precast gels available, please inquire!)
 
First ever native protein separation of alpha-synuclein, a protein believed to be responsible for Parkinson’s disease (PD), in cerebrospinal fluid (liquor, CSF) by isoelectric focusing
 



Alzheimer disease (AD)

Method: horizontal isoelectric focusing
Gel matrix: ProPhyl Air
Plastic carrier sheet: GelStick

Carrier amphlyte: Sepalyte CSF
Gel thickness: 750µm
CSF samples: undiluted

Application volume: 20µl
Separation distance: 8cm
Final voltage: 2800Volt
Stain: silver

Physical properties of ProPhyl Air


Metal screw nuts on top of gels
 
Left: ProPhyl Air, right: acrylamide 5%T,3%C

Swelling of the gels in water after 4 days
(casting molds are identical)
 
Left: ProPhyl Air, right: acrylamide 5%T,3%C

10 days drying at room temperature
(casting molds are identical)
         

 

New – ProPhyl Air Cat. No.: 54001 Quantity: 100ml/500ml Price: please inquire
New – Sepalyte CSF Cat. No.: 42025 Quantity: 10ml/25ml Price: please inquire


Future Outlook

With the help of ProPhyl Air it is now possible to discover native proteins that were previously undetectable with the conventional acrylamide gel matrix used worldwide for 60 years. Examples of such proteins are biomarkers for cancer, Parkinson’s disease (PD), Alzheimer’s (AD), and EPO. ProPhyl Air also exceeds the capabilities of the IPG matrix (2D electrophoresis) in certain areas, e.g. membrane proteins, because of excellent protein solubility in the gel matrix and superior performance in the first dimension of 2D. With ProPhyl Air, many proteins previously thought to be poorly soluble may no longer need detergents such as CHAPS, triton X-100 or NP-40. The use of denaturing 8M urea, which can falsify results in many ways, could thus become a thing of the past.
 
In some cases it is even possible to do detection without antibodies  (immunoblotting). The entire 2D ProPhyl Air electrophoresis process (with ampholytes, e.g. Sepalytes), including the pouring of gels (IEF / SDS) is completed in less than 4 hours, since a rehydration time of up to 12 hours and 8 hours of electrophoresis (IEF), as required with the ready-to-use IPG strips/gels is not necessary anymore. In addition, the current IPG strips/gels manufactured by various companies are rigid and therefore restrictive, since they allow the researcher no scope for variation of the cross linking of the IEF matrix (%T,%C). The comparative advantages of the new ProPhyl Air  gel matrix are its speed and flexibility.
 



How to cast your own horizontal IEF gel? 


IEF Gel: 265x193mm
thickness: 250µm
supporting sheet: GelStick™
ampholyte: Sepalyte pH 3-10™

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