Multiple sclerosis analysis by improved isoelectric focusing
with ProPhyl Air for the detection of oligoclonal bands (IgG) in cerebrospinal fluid (CSF – Liquor diagnostics)
Prof. Dr. med. Brigitte Wildemann / Prof. Dr. rer. nat. Jürgen Haas
Neurologie und Poliklinik
Otto-Meyerhof-Zentrum für Ambulante Medizin und Klinische Forschung
Im Neuenheimer Feld 350
CSF (Liquor diagnostics) examination in clinical research
As a service and supportive analysis, ProTec Bioseparation offers cost-effective examinations of CSF samples (liquor) for research laboratories.
This could be helpful for supportive protection in suspected cases (multiple sclerosis), as preclinical analysis or as a first guiding measure for neurodegenerative diseases.
This requires sending the most up-to-date sample material possible (CSF) and, in the event of suspected cases of MS, the corresponding serum.
The examination is carried out promptly after consultation and has the purpose to support the research laboratory with electrophoretic protein separations (reference material required) as the first orienting aid
What is Multiple Sclerosis?
Multiple sclerosis (MS) is a neuroinflammatory disease that affects myelin , a substance that makes up the membrane (called the myelin sheath) that wraps around nerve fibers (axons). Myelinated axons are commonly called white matter. Researchers have learned that MS also damages the nerve cell bodies, which are found in the brain’s gray matter, as well as the axons themselves in the brain, spinal cord, and optic nerve (the nerve that transmits visual information from the eye to the brain). As the disease progresses, the brain’s cortex shrinks (cortical atrophy).
The term multiple sclerosis refers to the distinctive areas of scar tissue (sclerosis or plaques) that are visible in the white matter of people who have MS. Plaques can be as small as a pinhead or as large as the size of a golf ball.
What causes MS?
The ultimate cause of MS is damage to myelin, nerve fibers, and neurons in the brain and spinal cord, which together make up the central nervous system (CNS). But how that happens, and why, are questions that challenge researchers. Evidence appears to show that MS is a disease caused by genetic vulnerabilities combined with environmental factors.
Although there is little doubt that the immune system contributes to the brain and spinal cord tissue destruction of MS, the exact target of the immune system attacks and which immune system cells cause the destruction isn’t fully understood.
Researchers have several possible explanations for what might be going on. The immune system could be:
- fighting some kind of infectious agent (for example, a virus) that has components which mimic components of the brain (molecular mimickry)
- destroying brain cells because they are unhealthy
- mistakenly identifying normal brain cells as foreign.
The last possibility has been the favored explanation for many years. Research now suggests that the first two activities might also play a role in the development of MS. There is a special barrier, called the blood-brain barrier, which separates the brain and spinal cord from the immune system. If there is a break in the barrier, it exposes the brain to the immune system for the first time. When this happens, the immune system may misinterpret the brain as “foreign.”
Diagnosis of multiple sclerosis
ProPhyl Air made a decisive contribution to the discovery of α-Synuclein in cerebrospinal fluid using the isoelectric focusing method
MVZ Labor Dr. Limbach & Kollegen GbR
Im Breitspiel 16
Different MS and negative liquor (CSF) samples applied side by side.
Unusual protein pattern in Liquor 1 (CSF)
|MVZ Labor Dr. Limbach & Kollegen GbR
Im Breitspiel 16
|Neurologie und Poliklinik
Ambulante Medizin und Klinische Forschung
Im Neuenheimer Feld 350
|SERVA Electrophoresis GmbH
Comparison between ProPhyl Air and Acrylamide matrix
ProPhyl Air matrix with Sepalyte CSF
Acrylamide matrix (supplier of precast gels)
What is multiple sclerosis?
Cause of multiple sclerosis
Symptoms of multiple sclerosis
multiple sclerosis diagnosis