Isoelectric focusing
Varietal purity determination of TRITICALE proteins using Sepalyte™ pH 3-6 WHEAT/TRITICALE
Differentiation between WHEAT and TRITICALE
In our tests we found:
a WHEAT-specific protein marker (not present in triticale) at the acid end pI < 4.0
a TRITICALE-specific protein marker (not present in wheat) at the base end pI > 5.0
(alternating left to right: five varieties of WHEAT and five of TRITICALE)
More tests will be done to verify the result !
Original Acid violet 17 stained gel
Separation of five TRITICALE varieties and five WHEAT varieties on one gel, using Sepalyte™ pH 3-6 WHEAT/TRITICALE carrier ampholyte.
(alternating left to right: five varieties of WHEAT and five of TRITICALE)
Staining : Acid Violet 17 C.I. 42640 (water soluble , needs no organic solvents)