NEW – NEW – NEW – NEW
Hydrophobic interaction isoelectric focusing (HIEF)
Novel gel matrix for the detection of Erythropoietin (EPO)
ProTec Bioseparation has succeeded in producing a novel gel matrix (ProPhyl Air), which makes it possible to detect Erythropoietin (EPO) by means of isoelectric focusing directly in the gel with a conventional Coomassie dye. The advantage of ProPhyl Air over conventional acrylamide is its large pore size with hydrophobic/hydrophilic gel properties that allow proteins to be separated in the gel without any addition of solubilizing agents such as urea or other non-ionic reagents. ProPhyl Air can be used without any change to the previous acrylamide IEF protocols and would therefore be ideal for the possible detection of recombinant EPO in urine or serum.
A comparison between Acrylamide and ProPhyl Air in isoelectric focusing electrophoresis
Conventional EPO determination according to the certified WADA method.
Agence française de lutte contre le dopage – Département des analyses
143 avenue Roger Salengro