Multiple Sclerosis

Intrathekal Detection of Immunglobulins in Liquor
(Multiple sclerosis analysis with the new ProPhyl Air matrix)


before evaluation (click for enlargement)


horizontal isoelectric focusing
gel matrix: ProPhyl Air
gel thickness: 750µm
carrier ampholyt: Sepalyte pH 3-10 (CSF)
plastic carrier support: GelStick polyester
sheet
stain: silver stain


Multiple sclerosis analysis by improved isoelectric focusing with ProPhyl Air for the detection of oligoclonal bands (IgG) in cerebrospinal fluid (CSF)
IgG antibodies from the cerebrospinal fluid and serum are applied side by side and appear as several (oligoclonal IgG) bands. Similar band patterns from both sources characterize the diffusion of the antibodies from the blood into the cerebrospinal fluid.
Oligoclonal bands that only occur in CSF indicate antibody formation in the central nervous system (CNS) in acute and chronic inflammatory diseases of the CNS. Cases 2,3,4,6,7 and 8 are typical for patients with multiple sclerosis. Only the cerebrospinal fluid has oligoclonal bands. The immunoglobulins shown by the bands were produced intrathecally. The presence of oligoclonal bands points to an inflammatory CNS process.
A typical feature of MS is that the inflammation can reoccur at different intervals in other parts of the nervous system. The name “multiple sclerosis” is derived from the fact that many (multiple) sites in the brain and spinal cord develop hardened scarring (sclerosis).

What is multiple sclerosis?
https://www.youtube.com/watch?v=SX7kHhmrelI

Cause of multiple sclerosis
https://www.dmsg.de/multiple-sklerose-infos/ms-verstehen/zusammenhaenge-erkennen/ursachen-der-ms/

Symptoms of multiple sclerosis
https://www.dmsg.de/multiple-sklerose-infos/ms-verstehen/vom-symptom-zur-therapie/symptome/

multiple sclerosis diagnosis
https://www.dmsg.de/multiple-sklerose-infos/ms-verstehen/vom-symptom-zur-therapie/diagnostik/

If you need more information: m.demharter@protec-biosep.de

samples:
Prof. Dr. med. Brigitte Wildemann / Prof. Dr. rer. nat. Jürgen Haas
Universitätsklinikum Heidelberg
Laborleiter AG Molekulare Neuroimmunologie
Otto-Meyerhof-Zentrum für Ambulante Medizin und Klinische Forschung
Im Neuenheimer Feld 350 

multiple sclerosis analysis

Multiple Sclerosis analysis
ProPhyl Air versus acrylamide

Multiple Sclerosis analysis with acrylamide

Multiple Sclerosis analysis with acrylamide
remark: reliable MS analysis not possible
Multiple Sclerosis analysis with acrylamide
remark: reliable MS analysis not possible